个人简介

葛台明，男，1966年1月出生，湖北武汉市人。博士、教授。第九届湖北省政治协商会议科技界委员，中国遗传学会、中国法医学会会员，湖北省生物工程学会理事，中国合格评定国家认可委员会（CNAL）实验室评审员、检查机构评审员。历任中国刑事科学技术协会特聘研究员，湖北警官学院客聘教授，公安部批青年专家人才库成员，湖北省法医学会物证委员会主任。

通讯地址：湖北省武汉市洪山区鲁磨路388号，中国地质大学（武汉）必赢唯一官方网站生物科学与技术系
邮政编码：430074
电话：027-67883160（办）
电子邮件：getm@263.net

主要学习培训简历
1981.9-1985.7在华中师范大学生物系学习，获理学学士学位。
1988.7参加河北省果树学会举办的“植物染色体研究技术学习班”的学习。
1990.9-1993.7在华中农业大学农学系作物遗传育种专业读硕士研究生，获农学硕士学位。
1994.9-1997.12在华中农业大学作物遗传改良国家重点实验室读博士，获农学博士学位。
2005.9在Applied Biosystems公司位于Rockville的培训基地参加“3100 and 3130 Genetic Analyzer User's Course”培训。

主要工作简历
1985.7-1999.12在湖北民族学院园艺系从事《遗传学》、《植物组织培养》、《微生物学》等课程的教学与科研工作。1992年任讲师，1997年任副教授。
2000.1-2006.10在湖北省公安厅刑事侦查总队刑事技术处从事人类法医学DNA鉴定及相关科研工作，2003年任副主任法医师，2005年任主任法医师。
2006.10-现在在中国地质大学（武汉）必赢唯一官方网站生物科学与技术系任教授。

主要研究兴趣
本人早期的研究兴趣主要集中在植物细胞工程及植物细胞遗传学领域，尤其是植物细胞工程领域。特别地，在植物体细胞杂交研究方面，首次在小麦中建立了一套完整的电融合技术体系，并第一个获得了“小麦＋多花黑麦草”的亚族间体细胞杂种及其成熟植株。调入中国地质大学（武汉）后，该领域的兴趣主要集中在利用植物细胞工程技术进行植物次生代谢产物的工厂化生产、植（作）物的品种改良、脱毒复壮，以及转基因等方面。
调入中国地质大学（武汉）后，本人将主要研究精力同时也放在了分子生态学及保育遗传学领域。利用中国地质大学（武汉）生物地质与环境地质国家重点实验室、湿地演化与生态恢复湖北省重点实验室等研究平台和技术优势，我们重点研究了微卫星标记的开发技术，并在该领域取得了重要进展，尤其在具有长重复基元（motif）的SSR标记开发方面取得了重要突破。结合中国地质大学（武汉）的学科优势和特色，今后的主要研究兴趣同时也将拓展至分子生态学、保护生物学（保育遗传学）、植物传粉生物学、群体遗传学、海洋生物学等学科。
总的来说，我的研究领域包括如下三个大的方面：
1、分子生态学及保育遗传学，包括利用SSR标记等分子生物学手段，研究动植物种群的群体遗传结构、濒危珍稀动植物遗传多样性及其保护生物学（保育遗传学）、植物传粉生物学、海洋生物分子生态及保育等方面。
2、植物细胞工程研究，主要从事利用植物细胞工程及相关技术手段，研究植物次生代谢产物的代谢机理及应用开发工作，同时也包括利用植物体细胞杂交、原生质体培养、花药及小孢子培养、茎尖脱毒与快繁等技术，进行作物的改良、野生植物资源的开发利用等工作。
3、法医物证及相关分子生物学检验技术研究。

科研工作简历
1. 国家自然科学基金重点项目（40930210）：长江中游全新世微生物对古温度和古水文条件的响应，2010.1-2013.12，主要参与者。
2. 武汉市科学技术局重点科技攻关项目（200920322149）：鳞茎类蔬菜脱毒试管种球生产技术研究，2009.1-2011.12，主持人。
3. 国家自然科学基金课题（批准号：39670385）：分离与鉴定小麦几丁质酶基因和葡聚糖酶基因及表达模式，1996.1-2000.12。排名第3，主要承担人。
4. 国家“九五”科技攻关课题（项目编号：96-001-01-06），真菌生长抑制基因介导的抗小麦赤霉病转基因植株，1996.1-2000.12。排名第3，主要承担人。
5. 湖北省自然科学基金课题（项目编号：94J）：富集锗植物细胞系的选育及培养研究，1994.6-1997.6，已完成。排名第3，主要完成人。
6. 湖北省教委重点项目（项目批准号：97A26）：试管苗玻璃化的防治和移栽技术研究，1998.1 - 2000.12，项目主持人，已完成。
7. 作物遗传改良国家重点实验室开放课题：小麦＋多花黑麦草不对称体细胞杂种育种价值及生物学特性研究，项目主持人，已完成。
8. 公安部科研项目(项目批准号：C20024229501)：牧羊犬10个STR基因座的研究，2001.9-2003.2，主要参与人。已完成并通过公安部组织的专家组鉴定为“国际先进水平”。
9. 公安部应用创新项目：纳米生物探针鉴别发案现场的样本DNA。与武汉大学合作，项目第二主持人。
10. 湖北省科技攻关计划项目(项目批准号：2006AA301B44-2)：纳米生物探针鉴别发案现场的样本DNA。与武汉大学合作，项目第一主持人。

近期发表的学术论文
SSR与分子生物学
1. 陶菲，童益琴，赵杰，南皓，杨立春，李江浩，葛台明*，2014。基于FIASCO技术开发芝麻SSR标记及其遗传多样性分析。湖北农业科学，已接受，待刊。
2. Yiqin Tong, Jie Zhao, Fei Tao, Jianghao Li, Taiming Ge*, 2014. Isolation and characterization of 16 highly polymorphic tetranucleotide microsatellite DNA markers in Paa spinosa. Biochemical Systematics and Ecology 57: 257-261.
3. LI Lei, BAO Origil, WANG Bin-Qi, GE Tai-Ming*, 2014. Using Modified FIASCO Protocol to Isolate Polymorphic Microsatellite Loci in Chinese Brake Fern (Pteris vittata L.): an Arsenic-Hyperaccumulating Plant. Plant Science Journal, 32(4):413-420. (English with Chinese abstract)
4. Li H, Huang J, Ding S, Ge T, 2014. Fifteen novel microsatellite markers for rock shell (Thais clavigera). Conservation Genet Resour, 6(1):123-125，负责SSR标记的开发
5. H. Li, T.M. Ge, S.X. Ding* and H.J. Wang, 2013. Novel tetranucleotide microsatellite markers for Chinese beard eel (Cirrhimuraena chinensis Kaup). Genetics and Molecular Research 12 (3): 2779-2782，负责SSR标记的开发
6. Huan Zheng, Gui-Rong Zhang, Shan-Shan Guo, Kai-Jian Wei*, Cong-Xin Xie, Tai-Ming Ge*, 2013. Isolation and characterization of fifteen polymorphic tetranucleotide microsatellite loci in Schizopygopsis younghusbandi Regan. Conservation Genet Resour, 5(4):1147-1149 (*: Corresponding Author，负责SSR标记的开发)
7. Yan Xu, Gui-rong Zhang, Shan-shan Guo, Xiang-zhao Guo, Kai-jian Wei*, Tai-ming Ge*, 2013. Isolation and characterization of fifteen polymorphic microsatellite loci in the threatened freshwater mussel Solenaia oleivora(Bivalvia: Unionidae). Biochemical Systematics and Ecology, 47:104-107 (*: Corresponding Author，负责SSR标记的开发)
8. 敖日格乐，贾　晓，葛台明，2009。SSR分子标记的开发策略概述。湖北民族学院学报（自然科学版），27(4)：462-467
9. 王海生、王　祥、葛台明、张文平、刘军训，2004，利用犬STR基因座检验认定犬精斑1例，刑事技术，(1):56
10. 王　祥、王海生、葛台明、童铁军，2003，腐败尸体阴道擦拭物中精子DNA检验1例，刑事技术，(6):10
次生代谢产物
1. 李江浩，葛台明*，洪岩，王焰新，王理。黄姜皂素生产工艺中的发酵机理与条件优化。天然产物研究与开发，已接受，待刊。
2. 李江浩, 葛台明*，2014。黄姜皂素提取工艺研究进展。山东农业大学学报（自然科学版），453):476-480
3. 李江浩，葛台明，2013。黄姜皂素清洁生产工艺研究进展。地球，(8)：302
4. 谷依露，高小峰，杨晓菁，葛台明，2012。不同物理、化学因子对致倦库蚊的引诱效果研究。中国媒介生物学及控制杂志，23(2):105-107
植物细胞工程
1. 赵　卓，舒礼佳，葛台明*，2011。不同浓度的NAA、6-BA对凤头姜试管苗生长的影响。湖北民族学院学报（自然科学版），29(3):278-281
2. T. M. Ge*, X. H. Lin, F. L. Qin, S. W. Yu, and Y. J. Yu, 2006. Protoplast electrofusion between common wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.) and regeneration of mature cybrids, In Vitro Plant, 42(2):179-187(9) (on line, DOI: 10.1079/IVP2005742)
3. Taiming Ge*, Yujun Yu, 2005. A powerful medium for strengthening and propagation of wheat regenerated plantlets, Electronic Journal of Biology, 1(3):33-35 (ISSN 1860-3122)
4. Ge Taiming, Yu Shunwu, Lin Xinghua, Qin Falan, Yu Yujun, Xie Yuefeng, 2002, Molecular identification of asymmetric somatic hybrids between wheat and Italian ryegrass, Chinese Science Bulletin, 47(24):2093-2095
5. 葛台明，余舜武，林兴华，秦发兰，余毓君，谢岳峰，2002，小麦＋多花黑麦草不对称体细胞杂种的分子鉴定，科学通报，47(19):1498-1499
6. 葛台明、章荣德、秦发兰、余毓君、谢岳峰，2000，冬小麦原生质体培养的胚状体直接发生，生物工程学报，16(5):609-613
7. 葛台明、章荣德、秦发兰、余毓君、谢岳峰，2000，多花黑麦草胚性悬浮系的建立及原生质体培养，华中农业大学学报，19(4):310-312
8. 葛台明、余毓君、谢岳峰、张端品、章荣德、秦发兰，1997，小麦＋多花黑麦草不对称体细胞杂交获得杂种再生植株，华中农业大学学报，16(2):105-111
9. 葛台明、余毓君，1997，小麦II型胚性愈伤组织的发现及培养特性研究，湖北民族学院学报，15(6):1-4
10. 葛台明、余毓君，1996，小麦花药培养的基因型和培养基效应研究，华中农业大学学报，15(5):410-413
11. 葛台明、余毓君，1995，高分化潜能小麦胚性悬浮系的建立与保持，武汉植物学研究，13(3):193-197
12. 朱根发、葛台明、余毓君，1995，小麦原生质体培养与植株再生的研究,华中农业大学学报, 14(4):315-321
13. 葛台明、余毓君，1994，小麦组织培养再生植株营养需求的研究，华中农业大学学报，13(4):344-348。

近期参加国际学术会议交流论文
1. Taiming GE, Xinghua LIN, Falan QIN, Shunwu YU, Yujun YU, Yuefeng XIE, 2004, Somatic Hybridization between Common Wheat and Italian Ryegrass, International Wheat Quality Conference -- From Molecular Improvement to Consumer Needs, Beijing, China. (Poster)
2. Ge Taiming, Yu Yujun, 1996, Obtainance of type II embryogenic callus and its cultured characteristics of wheat (Triticum aestivum L.), Abstracts of 2nd Asia-Pacific Conference on Plant Cell and Tissue Culture, Beijing, China. (Oral Presentation and poster)
3. Ge Taiming, Yu Yujun, 1996, A comparative studies on somaclonal and gametoclonal variation of wheat (Triticum aestivum L.), Abstracts of 2nd Asia-Pacific Conference on Plant Cell and Tissue Culture, Beijing, China. (Poster)
教学工作
主要从事《分子生物学》、《遗传学》、《细胞生物学》及《生物技术》等课程的教学。
GE Taiming - CURRICULUM VITAE
PERSONAL INFORMATION
FAMILY NAME: GE
FULL NAME: GE Taiming
GENDER: Male

CONTACT DETAILS
School of Environmental Studies,
China University of Geosciences, Wuhan,
Hubei, 430074, P.R. China
Tel: +86-27-67883160 (O); Fax: +86-27-87436235
E-mail: getm@263.net

CURRENT POSITION
Professor of Molecular and Cell Biology, Dept. of Bioscience and Biotechnology, School of Environmental Studies, China University of Geosciences (CUG).

EDUCATION
Ph.D. (Agronomy) from National Key Laboratory in Genetic Improvement of Crops, Huazhong Agricultural University, Dec. 1997.
MS (Agronomy) from Huazhong Agricultural University, July 1993.
BS (Biology) from Huazhong Normal University, July 1985.

EMPLOYMENT
1985-1992: Tutor of Biology, Dept. of Horticulture, Hubei University for Nationalities.
1992-1997: Lecturer of Biology, Dept. of Horticulture, Hubei University for Nationalities.
1997-2000: Associate Professor, Dept. of Horticulture, Hubei University for Nationalities.
2000-2006: Associate Professor [Associate Chief Criminalist (2000.1)], Professor [Chief Criminalist (2005.4)], Criminal Investigation Department, Public Security Bureau of Hubei Province.
2007-present: Professor, Department of Bioscience and Biotechnology, School of Environmental Studies, China University of Geosciences (CUG).

RESEARCH INTERESTS
My research interests include three main fields: Plant Cell Biology, Molecular Ecology and Human Identification (or other identification related to forensic analysis).

Cell Biology and Plant Cell Engineering
My earlier interests were Plant Cytogenetics and Plant Cell Engineering. Before I entered Huazhong Agricultural University as a postgraduate student, I analyzed karyotypes of some plants, so I am familiar with chromosomal analysis. When I was a PhD student, I developed the electrofusion techniques using the ‘donor-recipient’ model for the production of somatic hybrids/cybrids between common cultivated wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.). Considering that the two fusion partners are phylogenetically remote and are sexually incompatible, and both size and osmotic pressure of the protoplasts of fusion partners are different, the electrofusion difficulty can be anticipated.
Now, the main interests of these fields are focus on the following aspects: Cell suspension culture and secondary metabolites production, micropropagation and virus-elimination, in vitro production of haploids, transgenic plant regeneration.

Molecular Biology and Molecular Ecology
Thanks to the support from the National Key Laboratory of Biogeology and Environmental Geology (BGEG), and the Hubei Key Laboratory of Wetland Evolution and Ecological Restoration, currently, our lab has developed a novel strategy for isolating SSR markers, especially for isolating those with longer repeating unit (motif), for example, the trinucleotide repeating SSRs [e.g. (TTG)n] and tetranucleotide repeating SSRs [e.g. (GATA)n].The SSR markers with longer repeating unit are much better than those with shorter motif, because when amplifying the microsatellites with longer repeating unit, they displayed much weaker stutter bands, and easily to be analyzed. So the further interests should be focusing on the following:
1) Molecular Ecology and Conservation Biology of some endangered or economically important species (including marine species), especially using SSR markers to investigate evolutionary, ecological, and behavioural processes in these species. We hope that our research can help the Government to formulate conservation management plans for those species.
2) Reproductive Biology. According to Charles Robert Darwin’s theory about the formation of biodiversity through natural selection (and artificial selection on domestic species), all life is engaged in a perpetual struggle for survival and reproduction. Different organisms use different Gender Strategies to pass their genes to their offspring. By using SSRs (and other markers) to explore the mating (pollination) behaviour, I am also interested in trying to understand why the distribution areas of some endangered species were shrunken even without obvious disturbance of human being, and/or to understand the human impact on the endangered species.
3) New molecular techniques. For example, to isolate SSR markers in some non-model organisms, and to investigate the population structure of these organisms.

Human Identification
Based on my forensic experience, I am also interested in both analyzing the genotyping errors and investigating the distribution of some rare genes in human population. I am pleased to supervise some students in such field, especially those who come from the forensic system, or who wish to work in the field.
My interests also extend to the archaeological field. When I worked in the Criminal Investigation Department of Public Security Bureau of Hubei Province, I successfully extracted the sperm DNA in a body buried in wet sands for over 24 days (To my knowledge, this is the only successful example that had buried for a so long period). I also extracted DNA from skeletons buried for years. Thanks to the support from BGEG and the Criminal Investigation Department of Public Security Bureau of Hubei Province, I am also interested in using degraded DNA recovered from archived and archaeological samples to investigate past populations, in particular the influence of environmental change and exploitation on population dynamics and the evolution of species.

RESEARCH EXPERIENCES
1. NSFC (40930210): Microbial activities response to the paleo-temperature and paleo-hydrological changes during the Holocene in the middle reach of the Yangtze River, 2010.1-2013.12.
2. Key Technologies R & D Program of Science and Technology Bureau of Wuhan, Test-tube bulb production technique of garlic and Chinese onion (Allium chinense), 2009.1-2011.12
3. NSFC (39670385): Isolation and identification of the genes of chitinase and glucanase and their expression patterns, 1996.1-1999.12.
4. National Key Technologies R & D Program of China during the 9th Five-Year Plan Period (96-001-01-06): Scab resistant wheat from anti-fungi transgenic plants, 1996.1-2000.12.
5. Natural Science Foundation Project of Hubei (94J), Selection and culturing of germanium-rich cell lines of plant, 1994.6-1997.6.
6. Key project of Hubei Education Commission (97A26), Prevention of test-tube plantlets vitrification and their transplantation, 1998.1 - 2000.12.
7. Opening Project of National Key Laboratory of Crop Genetic Improvement: The characteristics and usage of somatic hybrid between wheat and Italian ryegrass, 1998.1 – 1999.12
8. Project of the Ministry of Public Security (C20024229501): 10 STR loci of shepherd dogs, 2001.9-2003.2.
9. Applying Innovation Project of the Ministry of Public Security (collaborated with Wuhan University): Identification of the Bio-samples from the Criminal Scene by Nanobioprobes.
10. Key Technologies R & D Program of Hubei Province (collaborated with Wuhan University, 2006AA301B44-2): Identification of the Bio-samples from the Criminal Scene by Nanobioprobes.

AWARDS
Year teacher in 2011, China University of Geosciences, Wuhan.
Year teacher in 2010, School of Environmental Studies, China University of Geosciences, Wuhan.
Year teacher in 1988, Hubei University for Nationalities.
Year teacher in 1987, Hubei University for Nationalities.

SELECED PUBLICATIONS
Plant Cell Engineering
1. T. M. Ge*, X. H. Lin, F. L. Qin, S. W. Yu, and Y. J. Yu, 2006, Protoplast electrofusion between common wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.) and regeneration of mature cybrids, In Vitro Cellular and Development Biology - Plant, 42(2):179-187(9) (on line, DOI: 10.1079/IVP2005742)
2. Ge Taiming*, Yu Yujun, A powerful medium for strengthening and propagation of wheat regenerated plantlets, Electronic Journal of Biology, 2005, 1(3): 33-35 (ISSN 1860-3122)
3. GE Tai-Ming, Zhang Rong-De, Qin Fa-Lan, YU Yu-Jun, XIE Yue-Feng, 2000. Direct Embryogenesis from Protoplast of Winter Wheat, Chinese Journal of Biotechnology, 16(5):609-613.
4. Ge Taiming, Zhang Rongde, Qin Falan, Yu Yujun, Xie Yuefeng, 2000. Establishment of Embryogenic Suspension and Protoplast Culture of Italian Ryegrass, Journal of Huazhong Agricultural University, 19(4):310-312.
5. Ge Taiming, Yu Yujun, Xie Yuefeng, Zhang Duanpin, Zhang Rongde, Qin Falan, 1997. Asymmetric somatic hybridization and plant regeneration between wheat and Italian ryegrass. Journal of Huazhong Agricultural University, 16(2):105-111.
6. Ge Taiming, Yu Yujun, 1997. Obtaining of type II embryogenic callus and its cultured characteristics of wheat (Triticum aestivum L.). Journal of Hubei Institute for Nationalities (natural science edition), 15(6):1-4.
7. Ge Taiming, Yu Yujun, 1996. Effects of genotypes and media on wheat anther culture. Journal of Huazhong Agricultural University, 15(5):410-413.
8. Ge Taiming, Yu Yujun, 1996. Obtaining of type II embryogenic callus and its cultured characteristics of wheat (Triticum aestivum L.). Abstracts of 2nd Asia-Pacific Conference on Plant Cell and Tissue Culture, I-14, Beijing, China.
9. Ge Taiming, Yu Yujun, 1996. Comparative studies on somaclonal and gametoclonal variation of wheat (Triticum aestivum L.). Abstracts of 2nd Asia-Pacific Conference on Plant Cell and Tissue Culture, V-20, Beijing, China.
10. Ge Taiming, Yu Yujun, 1995. Establishment of embryogenic suspension culture of wheat (Triticum aestivum L.). Journal of Wuhan Botanical Research, 13(3):193-197.
11. Zhu Genfa, Ge Taiming, Yu Yujun, 1995. Protoplast, culture and plantlet, s regeneration of wheat (Triticum aestivum L.). Journal of Huazhong Agricultural University, 14(4):315-321.
12. Ge Taiming, Yu Yujun, 1994. Studies on nutritional requirements of regenerated plantlets in wheat. Journal of Huazhong Agricultural University, 13(4):344-348.

Molecular Biology and Molecular Ecology
1. Yiqin Tong, Jie Zhao, Fei Tao, Jianghao Li, Taiming Ge*, 2014. Isolation and characterization of 16 highly polymorphic tetranucleotide microsatellite DNA markers in Paa spinosa. Biochemical Systematics and Ecology 57: 257-261.
2. LI Lei, BAO Origil, WANG Bin-Qi, GE Tai-Ming*, 2014. Using Modified FIASCO Protocol to Isolate Polymorphic Microsatellite Loci in Chinese Brake Fern (Pteris vittata L.): an Arsenic-Hyperaccumulating Plant. Plant Science Journal, 32(4):413-420. (English with Chinese abstract)
3. Li H, Huang J, Ding S*, Ge T, 2014. Fifteen novel microsatellite markers for rock shell (Thais clavigera). Conservation Genet Resour, 6(1):123-125, responsible for the isolation of the SSR loci
4. H. Li, T.M. Ge, S.X. Ding* and H.J. Wang, 2013. Novel tetranucleotide microsatellite markers for Chinese beard eel (Cirrhimuraena chinensis Kaup). Genetics and Molecular Research 12 (3): 2779-2782, responsible for the isolation of the SSR loci
5. Huan Zheng, Gui-Rong Zhang, Shan-Shan Guo, Kai-Jian Wei*, Cong-Xin Xie, Tai-Ming Ge*, 2013. Isolation and characterization of fifteen polymorphic tetranucleotide microsatellite loci in Schizopygopsis younghusbandi Regan. Conservation Genet Resour, 5(4):1147-1149 (*: Corresponding Author, responsible for the isolation of the SSR loci)
6. Yan Xu, Gui-rong Zhang, Shan-shan Guo, Xiang-zhao Guo, Kai-jian Wei*, Tai-ming Ge*, 2013. Isolation and characterization of fifteen polymorphic microsatellite loci in the threatened freshwater mussel Solenaia oleivora(Bivalvia: Unionidae). Biochemical Systematics and Ecology, 47:104-107 (*: Corresponding Author, responsible for the isolation of the SSR loci)
7. Ge Taiming, Yu Shunwu, Lin Xinghua, Qin Falan, Yu Yujun, Xie Yuefeng*, Molecular identification of asymmetric somatic hybrids between wheat and Italian ryegrass, Chinese Science Bulletin, 2002, 47(24):2093-2095

TEACHING EXPERIENCES
Lecturing on the following subjects since 1985:
1. Genetics
2. Molecular Biology (Molecular Genetics)
3. Cytogenetics
4. Cell Biology
5. Plant Cell Engineering (Plant Tissue Culture)
6. Microbiology